Time resolved fluorescence of flavin adenine dinucleotide
نویسندگان
چکیده
منابع مشابه
Combination with Flavin Adenine Dinucleotide*
The apoprotein of D-amino acid oxidase has been prepared by a new method involving dialysis of the holoenzyme against 1 M potassium bromide. The apoprotein prepared in this way has been found to have the same properties as that prepared by the classical acid-ammonium sulfate method. The effect of mixing apoprotein with flavin adenine dinucleotide has been followed by changes in absorbance at 49...
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An enzyme has been partially purified from brewer's yeast which catalyzes the reversible reaction between riboflavin phosphate and adenosine triphosphate (ATP) on the one hand and flavin-adenine dinucleotide (FAD) and inorganic pyrophosphate on the other in the presence of magnesium ions. ATP could not be replaced by adenosine diphosphate or adenosine-5-phosphate, and in the reverse reaction, i...
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Data obtained by means of proton magnetic resonance spectroscopy indicate that specific association of FMN occurs with AMP in aqueous solution, because much weaker interaction is observed with FMN of either GMP or CMP. A comparison of FAD with a 1:1 mixture of FMN and AMP suggests that the flavin is involved in an intramolecular hydrogen bonding with the adenine moiety of FAD. The temperature d...
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Antonie J. W. G. Visser,*,† Petra A. W. van den Berg,† Nina V. Visser,† Arie van Hoek,† Harrold A. van den Burg,‡ Derek Parsonage,‡ and Al Claiborne‡ MicroSpectroscopy Centre, Department of Biomolecular Sciences, Wageningen Agricultural UniVersity, P.O. Box 8128, NL-6700 ET Wageningen, The Netherlands, and Department of Biochemistry, Wake Forest UniVersity Medical Center, Winston-Salem, North C...
متن کاملTransient State Monitoring and Fluorescence Correlation Spectroscopy of Flavin Adenine Dinucleotide
Many human diseases including cancer have been associated with altered cellular metabolism and a changed oxygen consumption in cells. Fluorophores are sensitive to their local environment due to their long life times in transient dark states. A recent study successfully utilized this sensitivity to image differences in oxygen concentrations in cells using transient state (TRAST) microscopy toge...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1974
ISSN: 0014-5793
DOI: 10.1016/0014-5793(74)80307-8